Two-Component Response Regulators of Vibrio fischeri : Identification, Mutagenesis, and Characterization
نویسندگان
چکیده
منابع مشابه
Two-component response regulators of Vibrio fischeri: identification, mutagenesis, and characterization.
Two-component signal transduction systems are utilized by prokaryotic and eukaryotic cells to sense and respond to environmental stimuli, both to maintain homeostasis and to rapidly adapt to changing conditions. Studies have begun to emerge that utilize a large-scale mutagenesis approach to analyzing these systems in prokaryotic organisms. Due to the recent availability of its genome sequence, ...
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CcdB(Vfi) from Vibrio fischeri is a member of the CcdB family of toxins that poison covalent gyrase-DNA complexes. In solution CcdB(Vfi) is a dimer that unfolds to the corresponding monomeric components in a two-state fashion. In the unfolded state, the monomer retains a partial secondary structure. This observation correlates well with the crystal and NMR structures of the protein, which show ...
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Several bacterial response regulator proteins (CheY, NRI, PhoB, and OmpR) become phosphorylated in vitro when incubated with acetyl phosphate. In the presence of high levels of acetyl phosphate and Mg2+, CheY reached steady state phosphorylation in less than 30 s; NRI and PhoB reached steady state more slowly (t1/2 to steady state of 1.5 and > 15 min, respectively). A simple method was develope...
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Most Vibrio fischeri strains isolated from the Euprymna scolopes light organ carry plasmids, often including both a large (>40kb) plasmid, and one or more small (<12kb) plasmids. The large plasmids share homology with pES100, which is the lone plasmid in V. fischeri type strain ES114. pES100 appears to encode a conjugative system similar to that on plasmid R721. The small plasmids lack extensiv...
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Many bacteria developed a possibility to recognise aspects of their environment or to communicate with each other by chemical signals. An important strategy is the so-called quorum sensing (QS), a regulatory mechanism for the gene expression, where the bacteria measure their own cell density by means of this signalling pathway. One of the best-studied species using QS is the marine luminescent ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2007
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00242-07